Histological sections stained with safranin-O for CaP group. This area is the anterior of the aperture site in the tibia. T = tendon graft, B = bone, C = cartilage tissue, UF = uncalcified fibrocartilage-like tissue, CF = calcified fibrocartilage-like tissue. (a) Low-magnification image (x 40). The cartilage layer with glycosaminoglycan stained red was observed between the tendon graft and the bone. (b) Magnified views of the boxed part in (a) (x 400). The cartilage layer shows 2 distinct layers, namely, uncalcified fibrocartilage and calcified fibrocartilage layer without tidemarks.