Short and long terms healing of the experimentally transverse sectioned tendon in rabbits

Table 2 Ultrastructural fibrillar count and morphometric analysis of the injured tendons and their normal contralateral tendons on days 28 and 84 post injury and surgical intervention

	Variable	Four weeks post injury		Twelve weeks post injury
		Injured tendon	Normal tendon	Injured tendon	Normal tendon
Number of collagen fibrils at different range of collagen fibrils diameter	33-64 nm	617.50 ± 91.61	49.75 ± 6.13	378.00 ± 51.55	43.25 ± 7.36
	65-102 nm	Negative	9.50 ± 2.08	88.00 ± 25.85	17.50 ± 1.29
	103-153 nm	Negative	12.25 ± 2.50	Negative	14.50 ± 2.08
	154-256 nm	Negative	20.25 ± 2.21	Negative	10.00 ± 1.41
	257-307 nm	Negative	Negative	Negative	8.50 ± 0.577
	Total	617.50 ± 91.61	91.75 ± 1.70	466.00 ± 67.14	92.75 ± 8.65
Diameter of collagen fibrils at different range of collagen fibrils diameter
	33-64 nm	37.07 ± 4.75	38.20 ± 4.33	46.79 ± 4.37	37.73 ± 2.25
	65-102 nm	Negative	98.51 ± 2.65	70.50 ± 4.91	98.61 ± 3.04
	103-153 nm	Negative	149.56 ± 1.89	Negative	152.12 ± 0.74
	154-256 nm	Negative	234.39 ± 8.13	Negative	236.55 ± 9.07
	257-307 nm	Negative	Negative	Negative	278.28 ± 4.73
	Total	37.07 ± 4.75	177.50 ± 15.71	51.12 ± 4.11	193.25 ± 2.50
Number of elastic fibers
Number of elastic fibers	Elastic fibers	0.50 ± 0.57	Negative	0.50 ± 1.00	Negative
Collagen fibrils/Area²
Collagen fibrils/Area²	Density	51.67 ± 9.50	94.59 ± 3.00	84.56 ± 3.16	95.66 ± 2.77

Number of tissue section for each group = 10, number of ultra-micrograph in each tissue section = 5, pair sample t-Test and independent sample t-Test (P < 0.05), magnification = x39000. The collagen fibrils based on their diameter in the normal tendons were divided into 5 categories and the number of collagen fibrils and their diameter in each category were analyzed. Each data is the mean of the 50 ultra-micrographs. Normal contra lateral tendons contained 5 different ranges of collagen fibrils based on their diameter from 33 to 307 nm. The injured tendons on day 28 after injury had collagen fibrils in the range of 33-64 nm and the injured tendons on day 84 after injury had collagen fibrils in the range of 33-102 nm. The differences between groups were significant (P < 0.05). Number of the elastic fibers was not significant between groups (P > 0.05) and the collagen fibrils density (transvers area of the fibrils in the ultra-micrographs) was significantly different between groups (P < 0.05) and the injured tendons did not gain the normal density after 28 and 84 days post injury and surgical intervention.

ISSN: 2052-1847