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Table 2 Ultrastructural fibrillar count and morphometric analysis of the injured tendons and their normal contralateral tendons on days 28 and 84 post injury and surgical intervention

From: Short and long terms healing of the experimentally transverse sectioned tendon in rabbits

  Variable Four weeks post injury Twelve weeks post injury
   Injured tendon Normal tendon Injured tendon Normal tendon
Number of collagen fibrils at different range of collagen fibrils diameter 33-64 nm 617.50 ± 91.61 49.75 ± 6.13 378.00 ± 51.55 43.25 ± 7.36
65-102 nm Negative 9.50 ± 2.08 88.00 ± 25.85 17.50 ± 1.29
103-153 nm Negative 12.25 ± 2.50 Negative 14.50 ± 2.08
154-256 nm Negative 20.25 ± 2.21 Negative 10.00 ± 1.41
257-307 nm Negative Negative Negative 8.50 ± 0.577
Total 617.50 ± 91.61 91.75 ± 1.70 466.00 ± 67.14 92.75 ± 8.65
Diameter of collagen fibrils at different range of collagen fibrils diameter      
33-64 nm 37.07 ± 4.75 38.20 ± 4.33 46.79 ± 4.37 37.73 ± 2.25
65-102 nm Negative 98.51 ± 2.65 70.50 ± 4.91 98.61 ± 3.04
103-153 nm Negative 149.56 ± 1.89 Negative 152.12 ± 0.74
154-256 nm Negative 234.39 ± 8.13 Negative 236.55 ± 9.07
257-307 nm Negative Negative Negative 278.28 ± 4.73
Total 37.07 ± 4.75 177.50 ± 15.71 51.12 ± 4.11 193.25 ± 2.50
Number of elastic fibers      
Elastic fibers 0.50 ± 0.57 Negative 0.50 ± 1.00 Negative
Collagen fibrils/Area2      
Density 51.67 ± 9.50 94.59 ± 3.00 84.56 ± 3.16 95.66 ± 2.77
  1. Number of tissue section for each group = 10, number of ultra-micrograph in each tissue section = 5, pair sample t-Test and independent sample t-Test (P < 0.05), magnification = x39000. The collagen fibrils based on their diameter in the normal tendons were divided into 5 categories and the number of collagen fibrils and their diameter in each category were analyzed. Each data is the mean of the 50 ultra-micrographs. Normal contra lateral tendons contained 5 different ranges of collagen fibrils based on their diameter from 33 to 307 nm. The injured tendons on day 28 after injury had collagen fibrils in the range of 33-64 nm and the injured tendons on day 84 after injury had collagen fibrils in the range of 33-102 nm. The differences between groups were significant (P < 0.05). Number of the elastic fibers was not significant between groups (P > 0.05) and the collagen fibrils density (transvers area of the fibrils in the ultra-micrographs) was significantly different between groups (P < 0.05) and the injured tendons did not gain the normal density after 28 and 84 days post injury and surgical intervention.