Figure 1

Three Dimensional pattern of High Performance Liquid Chromatography in Boiogito. HPLC apparatus consisted of a Shimadzu LC 10A (analysis system software: CLASS-M10A ver. 1.64, Tokyo, Japan) equipped with a multiple wavelength detector (UV 200-400 nm)(Shimadzu SPD-M10AVP, diode array detector), an auto injector (Shimadzu CTO-10AC). HPLC conditions were described as follows: column, ODS (TSK-GEL 80TS, 250 × 4.6 mm i.d., TOSOH, Tokyo, Japan); eluent, (A) 0.05 M AcONH4 (pH 3.6) (B) 100% CH3CN. A linear gradient of 90% A and 10% B changing over 60 min to 0% A and 100% B was used. And 100% B was continued for 20 min.; temperature, 40 degrees celusius; flow rate, 1.0 mL/min. A granule was extracted with methanol (20 mL) under ultrasonication for 30 min., and was centrifuged at 3000 rpm for 5 min. The supernatant was filtrated with a membrane filter (0.45 μm) and then submitted for HPLC analysis (30 μL).