Cardiac hypertrophy is stimulated by altered training intensity and correlates with autophagy modulation in male Wistar rats

Table 1 Primers used for Semi quantitative-PCR analysis

Gene Symbol	Primer Sequence (5′ to 3′) Upper strand: sense Lower strand: antisense	Product Size (bp)	Annealing (°C)	Cycle	References
αMHC	GAGCAGGAGCTGATCGAGAC	151	60	35	[26]
αMHC	CCTCTGCGTTCCTACACTCC	151	60	35	[26]
PIK3CA	ACCTCAGGCTTGAAGAGTGTCG	137	59	35	[27]
PIK3CA	CCGTAAGTCGTCGCCATTTTTA	137	59	35	[27]
mTOR	CTGATGTCATTTATTGGCACAAA	170	57	35	[28]
mTOR	CAGGGACTCAGAACACAAATGC	170	57	35	[28]
LC3	GGTCCAGTTGTGCCTTTATTGA	153	59,5	35	[28]
LC3	GTGTGTGGGTTGTGTACGTCG	153	59,5	35	[28]
p62	CTAGGCATCGAGGTTGACATT	116	56	35	[29]
p62	CTTGGCTGAGTACCACTCTTATC	116	56	35	[29]
GAPDH	GTTACCAGGGCTGCCTTCTC	177	61	35	[30]
GAPDH	GATGGTGATGGGTTTCCCGT	177	61	35	[30]

ISSN: 2052-1847