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Dose
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Consumption of a NO3− dose ≥8 mmol
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NO3− form
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Provision of NO3− salts or vegetables, with NO3− content independently verified
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Study duration
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Longer duration (e.g., months-to-years) warranted
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Participant cohort
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‘At risk’ cohort studied (e.g., individuals with hypertension for studies exploring effects of NO3− on cardiovascular disease risk)
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Genetics/ microbiome
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Consider recruitment of T allele carriers with G894T polymorphism in the eNOS gene
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Microbiome
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Consider recruitment of individuals with greater abundance of NO3− reducing oral bacteria
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Mouthwash
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Avoidance of mouthwash prior to and during the study
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Dietary controls
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Avoidance of thiocyanate and sulphate rich foods in conjunction with NO3−
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Other lifestyle factors
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Avoidance of smoking
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Outcomes
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Inclusion of hard clinical endpoints (e.g., CVD or dementia incidence) to build upon promising findings on risk factors for these conditions
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