Table 1 Nucleotide sequences of primers used for qRT-PCR
From: Physical rehabilitation improves muscle function following volumetric muscle loss injury
Gene | Forward sequence | Reverse sequence | Amplicon length (BP) |
|---|---|---|---|
eMHC | 5′- TGGAGGACCAAATATGAGACG-3′ | 5′-CACCATCAAGTCCTCCACCT-3′ | 180 |
Collagen-1 | 5′-GACCAATGGGACCAGTCAGA-3′ | 5′-CTGGTGAACGTGGTGCAG-3′ | 123 |
TGF-β1 | 5′-GTCAGACATTCGGGAAGCA-3′ | 5′-CCAAGGTAACGCCAGGAAT-3′ | 138 |
SIRT-1 | 5′-GTTGACCTCCTCATTGTTATTGG-3′ | 5′-CGCAGTCTCCAAGAAGCTCT-3′ | 151 |
PGC-1α | 5′-CGTGTTCCCGATCACCATA-3′ | 5′-GTGTGCGGTGTCTGTAGTG-3′ | 108 |
18S | 5′-GGCCCGAAGCGTTTACTT-3′ | 5′-ACCTCTAGCGGCGCAATAC-3′ | 173 |