Fig. 1

General schematic of the study design and timeline After baseline echocardiography and treadmill acclimation, animals were randomly allocated to one of the four experimental groups: G1, HIIE 1 day following DOX injection; G2, HIIE 2 days following DOX injection; G3, HIIE 3 days following DOX injection; SED, sedentary (no access to exercise). T0, bolus DOX injection; endpoint echocardiography was performed on day 7 following DOX injection and all animals were sacrificed on day 9. DOX, doxorubicin; HIIE, high-intensity interval exercise