- Research article
- Open Access
- Open Peer Review
Agreement between test procedures for the single-leg hop for distance and the single-leg mini squat as measures of lower extremity function
BMC Sports Science, Medicine and Rehabilitation volume 10, Article number: 15 (2018)
Different test procedures are often used within performance-based measures, causing uncertainty as to whether results can be compared between studies. Thus, the aim of this study was to assess agreement between different test procedures for the single-leg hop for distance (SLHD) and the single-leg mini squat (SLMS), respectively, two commonly used tasks for assessing deficiency in lower extremity muscle function.
Twenty-three participants (20–42 years) with lower extremity injury performed the SLHD with arms free and with arms behind back, and the Limb Symmetry Index (LSI; injured leg divided by uninjured and multiplied by 100) was calculated. Another group of 28 participants (mean 18–38 years) performed five SLMSs at a pre-defined speed and maximum number of SLMSs during 30 seconds, and were visually observed and scored as either having a knee-over-foot or a knee-medial-to-foot position (KMFP).
No systematic difference between test procedures for the LSI of the SLHD was noted (p=0.736), Cohen’s kappa = 0.42. The Bland & Altman plot showed wide limits of agreement between test procedures, with particularly poor agreement for participants with abnormal LSI (<90%). Ten participants were scored as having a KMFP during five SLMSs at a predefined speed, while five had a KMFP during maximum number of SLMSs during 30 seconds (p=0.063, Cohen’s kappa = 0.56).
The moderate agreement between the two test procedures for the SLHD and the SLMS, respectively, indicate that results from these different test procedures should not be compared across studies. SLHD with arms behind back, and five SLMSs at a pre-defined speed, respectively, were the most sensitive procedures to detect individuals with poor functional performance.
Performance-based measures (PBM) are commonly used as screening tools to identify athletes with higher risk of injury [1,2,3] as well as to assess deficiency in muscle function, and effects of interventions on muscle function, in people with lower extremity injury [4,5,6,7]. PBMs have the advantage that they are easy to administer in the clinical setting and in research because they require little equipment and are quickly accomplished. Several, more or less evidence-based PBMs, such as the single leg squat and different jump landing tasks are available in the clinical and sporting settings [8,9,10,11]. Two valid  and reliable [8, 12] and commonly used PBMs are the single-leg mini squat (SLMS), resembling activity of daily living, for visual assessment of medio-lateral knee position [12, 13], and the single-leg hop for distance (SLHD) [5, 14], resembling more demanding sporting activity. However, different test procedures are often used within each task, causing uncertainty as to whether results can be compared between studies [5, 12,13,14].
The SLHD is performed either with arms behind back or with arms free during the execution of the hop, and the Limb Symmetry Index (LSI) is typically used to assess differences in hop distance between the injured and uninjured legs . The knee position relative to the foot is assessed and scored during either the performance of five SLMSs at a predefined speed  or during the performance of maximum number of SLMSs during 30 seconds . The aims of the current study were to assess agreement between the different test procedures within the tasks for the SLHD and the SLMS, respectively and to determine if any of the within task procedure is more sensitive to detect individuals with worse functional performance than the other.
As a sample of convenience, two groups of participants were included. Since the LSI between the injured and non-injured legs is most frequently used when evaluating the SLHD, one group with lower extremity injury performed the SLHD (group 1). The SLMS, on the other hand, is commonly used as a screening task for detecting undesirable knee movement patterns in healthy individuals and thus, another group of non-injured individuals performed the SLMS (group 2). Patient demographics were collected prior to the assessment of the functional tasks. The participants were recruited among physical therapy students at the Faculty of Medicine, Lund University, Sweden and screened for eligibility by the assessors (physical therapists). The Advisory Committee for Research Ethics in Health Education at the Faculty of Medicine, Lund University approved the study (VEN 78-10, VEN 91-10) and the participants gave their written informed consent.
For the assessment of the SLHD, 23 participants (16 women) were included. The inclusion criteria were: i) 18 to 45 years, ii) any injury to the hip, knee or foot causing perceived functional limitations (functional limitations are similar in people with an injury or disease [4, 15]) representing at least monthly awareness of hip/knee/foot problem (subscale quality of life, question 1) in the Hip dysfunction and Osteoarthritis Outcome Score (HOOS), Knee injury and Osteoarthritis Outcome Score (KOOS), or Foot and Ankle Outcome Score (FAOS), as appropriate [16,17,18] , iii) ability to perform a single-leg hop for distance. The HOOS, KOOS and FAOS are valid and reliable questionnaires, developed to assess patients’ opinions about their hip, knee and foot, respectively, in patients with and without osteoarthritis [18,19,20]. Volunteers were excluded if they had other injuries or diseases overriding the hip, knee or foot symptoms, or if they, for any reason, were unable to perform the SLHD. Three participants had bilateral lower extremity injury. In those cases, the leg causing most perceived problems was determined as injured. All participants were physically active  (Table 1). Their KOOS/HOOS/FAOS scores were comparable to other cohorts with lower extremity injury [16, 18, 22], suggesting that this group was representative for the purpose of this study.
For the assessment of the SLMS, 28 physically active participants (15 women), 18 years or older were included (Table 1). People with any injuries limiting their daily activities and/or their ability to complete the squatting test were excluded.
Single-leg hop for distance (SLHD)
The participants performed the SLHD with their hands placed behind their back , and with the arms free  aiming at a more functional execution of the hop, in a randomized  order (Fig. 1). The participant stood on the tested leg with the toes behind a marked line, and with the other leg lifted from the floor by flexing the knee. The participants were told to hop as far as possible, taking off and landing on the same foot, maintaining their balance for about 2-3 seconds. The test was performed three times with each leg, alternating the right and left leg. The hop distance (cm) was measured from toe in the starting position to heel in the landing position with a measuring tape by the same examiner. If the participant improved more than 10 cm between the second and third hop, additional hops were performed until an increase of less than 10 cm was measured. One trial hop preceded the measurements. The participants wore their own shoes, e.g., sneakers.
Single-limb mini squat (SLMS)
The participants performed five SLMSs at a pre-defined speed of 20 squats/min (i.e., 3 seconds from starting position to the knee flexion position and back to the starting position)  and maximum number of single-limb squats during 30 seconds . The tasks were performed in a randomized order, to avoid any fatiguing effects. The right leg was tested in all participants, and the participants were barefoot during the test . The procedure for the SLMSs was as follows: A “T” was marked with tape on the floor. The patient stood with the long axis of the foot aligned to the stem of the “T”; the second toe placed on the stem. The other leg was kept with the hip in slight flexion and the knee in about 80 degrees of flexion. Finger-tip support for balance was provided by the examiner. The participant was then asked to look down and bend his/her knee, without bending forward from the hip, until he/she no longer could see the line along the toes (corresponding to about 50 degrees of knee flexion), and then return to extension. Three practice trials preceded the SLMS at a pre-defined speed and a 10 second trial preceeded the SLMSs during 30 seconds. The examiner were placed in front of the subject [12, 13] and thhe participant was scored as either having a knee over foot position (KOFP) or a knee medial to foot position (KMFP). A KOFP was scored when the knee was well aligned over or lateral to the 2nd toe in three or more of five trials for the SLMSs at the pre-defined speed, and in the majority of the maximum number of SLMSs during 30 seconds. A KMFP was scored when the knee was placed medial to the 2nd toe (Fig. 2). Two examiners separately assessed and scored the participants’ knee position relative to the foot in real time. After each participant was assessed, the two examiners discussed the scoring of the knee position and a consensus was used in the analysis. The examiners were well trained by an experienced examiner, from pilot testing preceding the present study. The participants were not told that the knee position relative to the foot was assessed during the tests.
For the SLHD, the mean value of the three hops was used in the analysis. Analysis without the 3 participants with bilateral injury did not alter the results; therefore, data is given for all participants. The LSI value, calculated by dividing the result for the injured leg by that of the uninjured leg and multiplying by 100, was used for comparison between the two test procedures (hands behind back vs arms free during the execution of the hop). The mean difference and 95% CI, and “Bland & Altman plots” (scatter plots of the difference between the methods against their mean) with limits of agreement (LOA) [26, 27] were used for assessing agreement between the two test procedures. The “Bland and Altman plot” is the preferred statistical test when assessing agreement between different test procedures and shows systematic differences as well as heteroscedasticity, i.e., larger variability in one end of the measuring scale [26, 27]. In our data for the SLHD, the variability was much larger when the LSI was low. A logarithmic transformation could possibly decrease this relationship . However, logging the data for the Bland & Altman plot did not change the result, and, therefore, the original data is presented. The LSI was also dichotomized into two groups: one group including the participants that had an LSI of 90 or above (normal LSI), and one group that had an LSI below 90 (abnormal LSI) . Mc Nemar’s test was used to test the proportion of normal vs abnormal LSI for the SLHD, and KMFP vs KOFP for the SLMS, between the test procedures. Cohen’s Kappa coefficient was used as a measure of agreement between the procedures. The following thresholds for the Cohens’s Kappa coefficient were used; <0.00 poor agreement, 0.00-0.20 slight agreement, 0.21-0.40 fair agreement, 0.41-0.60 moderate agreement, 0.61-0.80 substantial agreement, 0.81-1.00 almost perfect agreement . Pearsons’ correlation coefficient and Spearmans’ rank correlation coefficient were used as appropriate to assess possible associations between demographics and performance during the SLHD and SLMS, respectively. A level of p≤0.05 was chosen to indicate statistical significance.
There were no associations between demographics and medio-lateral knee position for the two procedures for the SLMS. A lower activity level was associated with a higher LSI for arms behind back (r=0.41, p=0.049) during the SLHD.
Single-leg hop for distance
The participants hopped a shorter distance with their injured leg than their uninjured leg, with hands behind their back and with arms free (p≤0.004) (Table 2), indicating functional deficiency. There was no systematic difference between the two test procedures for the LSI (Table 2). The Bland & Altman plot revealed a wide LOA between the two procedures with particularly poor agreement when participants had abnormal LSI (Fig. 3). Cohen’s kappa was 0.416 (95%CI 0.010 to 0.822, p=0.033). Cross-tabulation showed poor agreement between the procedures for the proportion of participants with abnormal LSI (p=0.375) (Table 3).
Single-limb mini squat
Ten participants were scored as having a KMFP during the performance of five SLMSs at a predefined speed of 20 squats per minute, and five participants during the performance of maximum number of SLMSs during 30 seconds (p=0.063) (Table 4). Cohen’s kappa was 0.56 (95% CI 0.251 to 0.875, p=0.001), reflecting the agreement between the two test procedures.
Nearly twice as many participants had abnormal LSI with arms behind back (30%) compared to the procedure with arms free (17%) during the SLHD, indicating that the hop test performed with arms behind back is better at detecting individuals with functional limitations than arms free. The Bland and Altman plot showed that the agreement between the procedures seems to be good when the participants had an LSI close to normal, but worse when the participants had lower LSI. This indicates that the result from studies using these two different procedures (arms behind back vs arms free) should not be compared.
A well-known limitation of the LSI is that an injury may affect also the uninjured leg. That is, a small difference between the injured and uninjured legs may be due to impaired function of both legs, causing an overestimation of the LSI and, consequently, function of the knee . This misinterpretation may lead to a return to sport too early after injury and, thus an increased risk of re-injury . Despite this limitation, results from several studies suggest that the LSI is a useful measure. Longitudinal studies report that a low LSI predicts poor long-term outcomes, such as worse perceived function [30, 31], less likelihood of returning to sports , and OA development . The LSI is also a useful variable in order to minimize the influence of multiple testers and different testing devices , and it is widely used as a return to sport criterion . However, when using the LSI as a measure of function after injury, the result from this study indicates that performing the tasks with arms behind back may be more demanding and thus more sensitive in detecting muscle function deficiency compared to arms free in this group of patients. Also, since this study included participants with different injuries, i.e., hip, knee and foot injuries, this result may be generalizable to patients with a wide range of musculoskeletal complaints. Further studies may reveal if arms behind back is the most appropriate SLHD protocol for predicting other outcomes as well, such as return to sport and perceived function in patients with injury to the lower extremity.
Twice as many participants were scored as having a KMFP during the performance of five SLMSs at a predefined speed of 20 squats per minute (40%), than during the performance of maximum number during 30 seconds (20%). The difference did not reach statistical significance (p=0.063). However, it indicates that five SLMSs at a slow speed may be better at detecting individuals with functional limitations, and the difference was considered clinically relevant for the purpose of this study. Also, the kappa value of 0.56 suggests only a moderate agreement between the two test procedures implying that these procedures should not be used interchangeably. Taken together, this suggests that results from studies using these different test procedures should not be compared.
A medially placed knee in relation to the foot is more common in women compared with men  and is, thus, suggested to be associated with the higher knee injury risk observed in females . This altered movement pattern is also reported to be related to worse perceived knee function [37,38,39], worse knee confidence  and worse hop performance  in patients with lower extremity injury, implying that this is a valuable measure of functional performance. The observed moderate correlation between postural orientation errors (measurement of joints in relation to each other) and hop tests , indicate that these measures encompass different aspects of performance and that both can be used to obtain a complete picture of the patient’s function. It may be valuable in future studies to record the performance of the SLMS during 30 seconds on video, so that the knee position in relation to the foot can be viewed repeatedly and/or in slow motion . This would determine whether the difference in our study is due to the difficulty of assessing the knee position relative to the foot in real time or if 5 slow squats is a more sensitive test for detecting a KMFP during a SLMS.
This study has some limitations. Lower Grimby activity level was associated with higher LSI during the arms behind back procedure for the SLHD (r=0.41, p=0.049). However, the correlation coefficient between the activity level and the SLHD with the arms free was quite similar (r=0.35, p=0.104) but did not reach statistical significance. Since the activity level seems to affect the LSI in a similar manner for both procedures, we do not believe that this have had an effect on the results in this study. Also, since this is a cross-sectional study, we cannot draw any conclusions regarding causal relationship between activity level and LSI during the hop tests. Another limitation is the small sample size. A sample of at least 50 individuals is recommended in agreement studies . Even though the result from this study seems to be sufficiently evident, it is possible that a larger sample may have altered the result.
Comparisons should not be made between groups of participants with lower extremity injury for the LSI of the SLHD when the different test procedures arms free or behind back during the performance of the hop have been used. Neither should results for medio-lateral knee position during the performance of the two SLMS protocols be compared. SLHD with arms behind back and 5 SLMS at a pre-defined speed may be preferred to identify individuals with worse function as assessed by hop distance and medio-lateral knee position, respectively.
Foot and ankle outcome score
Hip dysfunction and osteoarthritis outcome score
Knee medial to foot position
Knee over foot position
Knee injury and osteoarthritis outcome score
Limits of agreement
Limb symmetri index
Performance based measures
Single leg hop for distance
Single leg mini squat
Goossens L, Witvrouw E, Vanden Bossche L, et al. Lower eccentric hamstring strength and single leg hop for distance predict hamstring injury in PETE students. Eur J Sport Sci. 2015;15(5):436–42.
Myer GD, Ford KR, Palumbo JP, et al. Neuromuscular training improves performance and lower-extremity biomechanics in female athletes. J Strength Cond Res. 2005;19(1):51–60.
Kodesh E, Shargal E, Kislev-Cohen R, et al. Examination of the Effectiveness of Predictors for Musculoskeletal Injuries in Female Soldiers. J Sports Sci Med. 2015;14(3):515–21.
Witchalls JB, Newman P, Waddington G, et al. Functional performance deficits associated with ligamentous instability at the ankle. J Sci Med Sport. 2013;16(2):89–93.
Thomee R, Kaplan Y, Kvist J, et al. Muscle strength and hop performance criteria prior to return to sports after ACL reconstruction. Knee Surg Sports Traumatol Arthrosc. 2011;19(11):1798–805.
Pattyn E, Mahieu N, Selfe J, et al. What predicts functional outcome after treatment for patellofemoral pain? Med Sci Sports Exerc. 2012;44(10):1827–33.
Ericsson YB, Dahlberg LE, Roos EM. Effects of functional exercise training on performance and muscle strength after meniscectomy: a randomized trial. Scand J Med Sci Sports. 2009;19(2):156–65.
Gustavsson A, Neeter C, Thomee P, et al. A test battery for evaluating hop performance in patients with an ACL injury and patients who have undergone ACL reconstruction. Knee Surg Sports Traumatol Arthrosc. 2006;14(8):778–88.
Krosshaug T, Steffen K, Kristianslund E, et al. The Vertical Drop Jump Is a Poor Screening Test for ACL Injuries in Female Elite Soccer and Handball Players: A Prospective Cohort Study of 710 Athletes. Am J Sports Med. 2016;
Reid A, Birmingham TB, Stratford PW, et al. Hop testing provides a reliable and valid outcome measure during rehabilitation after anterior cruciate ligament reconstruction. Phys Ther. 2007;87(3):337–49.
Nae J, Creaby MW, Cronstrom A, et al. Measurement properties of visual rating of postural orientation errors of the lower extremity - A systematic review and meta-analysis. Phys Ther Sport. 2017;
Ageberg E, Bennell KL, Hunt MA, et al. Validity and inter-rater reliability of medio-lateral knee motion observed during a single-limb mini squat. BMC Musculoskelet Disord. 2010;11:265.
Örtqvist M, Mostrom EB, Roos EM, et al. Reliability and reference values of two clinical measurements of dynamic and static knee position in healthy children. Knee Surg Sports Traumatol Arthrosc. 2011;19(12):2060–6.
Kroman SL, Roos EM, Bennell KL, et al. Measurement properties of performance-based outcome measures to assess physical function in young and middle-aged people known to be at high risk of hip and/or knee osteoarthritis: a systematic review. Osteoarthritis Cartilage. 2014;22(1):26–39.
Roos PE, Button K, Sparkes V, et al. Altered biomechanical strategies and medio-lateral control of the knee represent incomplete recovery of individuals with injury during single leg hop. J Biomech. 2014;47(3):675–80.
Nilsdotter AK, Lohmander LS, Klassbo M, et al. Hip disability and osteoarthritis outcome score (HOOS)--validity and responsiveness in total hip replacement. BMC Musculoskelet Disord. 2003;4:10.
Roos EM, Roos HP, Lohmander LS, et al. Knee Injury and Osteoarthritis Outcome Score (KOOS)--development of a self-administered outcome measure. J Orthop Sports Phys Ther. 1998;28(2):88–96.
Roos EM, Brandsson S, Karlsson J. Validation of the foot and ankle outcome score for ankle ligament reconstruction. Foot Ankle Int. 2001;22(10):788–94.
Klassbo M, Larsson E, Mannevik E. Hip disability and osteoarthritis outcome score. An extension of the Western Ontario and McMaster Universities Osteoarthritis Index. Scand J Rheumatol. 2003;32(1):46–51.
Collins NJ, Prinsen CA, Christensen R, et al. Knee Injury and Osteoarthritis Outcome Score (KOOS): systematic review and meta-analysis of measurement properties. Osteoarthritis Cartilage. 2016;24(8):1317–29.
Grimby G. Physical activity and muscle training in the elderly. Acta Med Scand Suppl. 1986;711:233–7.
Ageberg E, Forssblad M, Herbertsson P, et al. Sex differences in patient-reported outcomes after anterior cruciate ligament reconstruction: data from the Swedish knee ligament register. Am J Sports Med. 2010;38(7):1334–42.
Tegner Y, Lysholm J, Lysholm M, et al. A performance test to monitor rehabilitation and evaluate anterior cruciate ligament injuries. Am J Sports Med. 1986;14(2):156–9.
Zätterström R, Fridén T, Lindstrand A, et al. Rehabilitation following acute anterior cruciate ligament injuries--a 12-month follow-up of a randomized clinical trial. Scand J Med Sci Sports. 2000;10(3):156–63.
Kim J, Shin W. How to do random allocation (randomization). Clin Orthop Surg. 2014;6(1):103–9.
Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986;1(8476):307–10.
Bland JM, Altman DG. Measuring agreement in method comparison studies. Stat Methods Med Res. 1999;8(2):135–60.
Landis JR, Koch GG. The measurement of observer agreement for categorical data. Biometrics. 1977;33(1):159–74.
Wellsandt E, Failla MJ, Snyder-Mackler L. Limb Symmetry Indexes Can Overestimate Knee Function After Anterior Cruciate Ligament Injury. J Orthop Sports Phys Ther. 2017;47(5):334–8.
Logerstedt D, Grindem H, Lynch A, et al. Single-legged hop tests as predictors of self-reported knee function after anterior cruciate ligament reconstruction: the Delaware-Oslo ACL cohort study. Am J Sports Med. 2012;40(10):2348–56.
Grindem H, Logerstedt D, Eitzen I, et al. Single-Legged Hop Tests as Predictors of Self-reported Knee Function in Nonoperatively Treated Individuals With Anterior Cruciate Ligament Injury. Am J Sports Med. 2011;
Ardern CL, Webster KE, Taylor NF, et al. Return to the preinjury level of competitive sport after anterior cruciate ligament reconstruction surgery: two-thirds of patients have not returned by 12 months after surgery. Am J Sports Med. 2011;39(3):538–43.
Pinczewski LA, Lyman J, Salmon LJ, et al. A 10-year comparison of anterior cruciate ligament reconstructions with hamstring tendon and patellar tendon autograft: a controlled, prospective trial. Am J Sports Med. 2007;35(4):564–74.
Ericsson YB, Roos EM, Frobell RB. Lower extremity performance following ACL rehabilitation in the KANON-trial: impact of reconstruction and predictive value at 2 and 5 years. Br J Sports Med. 2013;47(15):980–5.
Cronstrom A, Creaby MW, Nae J, et al. Gender differences in knee abduction during weight-bearing activities: A systematic review and meta-analysis. Gait Posture. 2016;49:315–28.
Hewett TE, Myer GD, Ford KR, et al. Biomechanical measures of neuromuscular control and valgus loading of the knee predict anterior cruciate ligament injury risk in female athletes: a prospective study. Am J Sports Med. 2005;33(4):492–501.
Trulsson A, Roos EM, Ageberg E, et al. Relationships between postural orientation and self reported function, hop performance and muscle power in subjects with anterior cruciate ligament injury. BMC Musculoskelet Disord. 2010;11:143.
Salsich GB, Graci V, Maxam DE. The effects of movement pattern modification on lower extremity kinematics and pain in women with patellofemoral pain. J Orthop Sports Phys Ther. 2012;42(12):1017–24.
Flosadottir V, Roos EM, Ageberg E. Muscle function is associated with future patient-reported outcomes in young adults with ACL injury. BMJ Open Sport & Exercise Medicine. 2016;2(1)
Ageberg E, Roos EM. The Association Between Knee Confidence and Muscle Power, Hop Performance, and Postural Orientation in People With Anterior Cruciate Ligament Injury. J Orthop Sports Phys Ther. 2016;46(6):477–82.
Nae J, Creaby MW, Cronström A, et al. Measurement properties of visual rating of Postural Orientation Errors (POEs) of the lower extremity - A systematic review and meta-analysis. Physical Therapy in Sport. 2017;27:52–64.
Altman DG. Practical statistics for medical research. London: Chapman and Hall; 1991.
We thank physical therapists Ida Areskoug, Erik Gillenius, Maria Klanglund, and Ida Reimer for collecting data and Tommy Schyman for statistical advice.
This research was funded by the Faculty of Medicine, Lund University.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Ethics approval and consent to participate
The Advisory Committee for Research Ethics in Health Education at the Faculty of Medicine, Lund University approved the study (VEN 78-10, VEN 91-10) and the participants gave their written informed consent.
Consent for publication
The persons in the pictures were not included in the data collection, but they gave their written consent to publish the images.
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Ageberg, E., Cronström, A. Agreement between test procedures for the single-leg hop for distance and the single-leg mini squat as measures of lower extremity function. BMC Sports Sci Med Rehabil 10, 15 (2018) doi:10.1186/s13102-018-0104-6
- Lower extremity
- performance-based measures
- hop performance
- postural orientation
- reproducibility of results